Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Sorcin regulates alveolarization and airway tissue remodeling during lung morphogenesis
doi: 10.1007/s00018-025-05870-y
Figure Lengend Snippet: Altered protein expression and EGFR signaling in Sorcin-deficient lung development A ) Confocal microscopy of 3-week-old lung tissue from WT and Sri⁻ / ⁻ mice. Sri⁻ / ⁻ lung tissue shows reduced SP-B (red) expression compared to WT. Scale bars, 20 μm B ) Western blot analysis of lung lysates from 3-week-old WT and Sri⁻ / ⁻ mice. Sri⁻ / ⁻ mice exhibit decreased EGFR, PANRAS and RAB5C protein levels compared to WT. Densitometry quantification is shown (mean ± SEM). ** p < 0.01 (Student’s t-test), *** p < 0.001 (determined by Student’s t-test) C-E ) Confocal microscopy of 3-week-old lung sections: WT mice show epithelial localization of EGFR (green) (C), RAB5C (green) (D) and PANRAS (red) protein (E). In Sri⁻ / ⁻ mice, these proteins are significantly reduced. Scale bars, 20 μm
Article Snippet: Then, sections were blocked in 5% goat serum for 1 h and incubated overnight at 4 °C with primary antibody rabbit polyclonal EPCAM/CD326 (1:100 in 1%BSA-PBS solution) (Proteintech, #21050-1-AP), rabbit polyclonal SFTPB (1:100 in 1%BSA-PBS solution) (Thermo Fisher Scientific, # PA542000 ), rabbit polyclonal E-cadherin (1:100 in 1%BSA-PBS solution) (Proteintech, #20874-1-AP), mouse monoclonal E-cadherin (1:100 in 1%BSA-PBS solution) (BD Bioscience, #610181), mouse monoclonal α-Smooth Muscle Actin (1:100 in 1%BSA-PBS solution) (Sigma-Aldrich, #A5228), rabbit polyclonal EGFR (1:100 in 1%BSA-PBS solution) (Proteintech, #30847-1-AP) rabbit polyclonal RAB5C (1:100 in 1%BSA-PBS solution) (Thermo Fisher Scientific, #PA5101828), mouse monoclonal PANRAS (Ab3) (1:100 in 1%BSA-PBS solution) (Sigma-Aldrich, #OP40) followed by incubation with Alexa fluor 488 (rabbit)-conjugated secondary antibodies (1:500 in 1%BSA-PBS solution) (Thermo Fisher Scientific).
Techniques: Expressing, Confocal Microscopy, Western Blot
